Description
Optical properties
λ abs = 663 nm
ε max = 1.25×10 5 M -1 cm -1
λ fl = 680 nm
n fl = 30 %
τ fl = 1.8 ns
CF 260 = ε 260 /ε max = 0.24
CF 280 = ε 280 / ε max = 0.08
ATTO 655 belongs together with ATTO 680 and ATTO 700 to a new generation of fluorescent labels.
Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, exceptionally high stability towards atmospheric ozone, and very good water solubility. Thus ATTO 655 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS) , fluorescence in-situ hybridization (FISH and many more.
ATTO 655 is a zwitterionic dye. After coupling to a substrate the dye moiety is electrically neutral. ATTO 655 is a strong electron acceptor. Its fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc. The fluorescence is excited most efficiently in the range 640 - 660 nm . A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.
