Description
Optical properties
λ abs = 700 nm
ε max = 1.25×10 5 M -1 cm -1
λ fl = 716 nm
n fl = 25 %
τ fl = 1.6 ns
CF 260 = ε 260 /ε max = 0.26
CF 280 0 = ε 280 /ε max = 0.41
ATTO 700 belongs together with ATTO 655 and ATTO 680 to a new generation of fluorescent labels.
Characteristic features of the label are strong absorption, high fluorescence quantum yield, good water solubility, and high thermal and photo-stability. ATTO 700 is a zwitterionic dye. After coupling to a substrate the dye moiety is electrically neutral.
ATTO 700 is a strong electron acceptor. Its fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc. The fluorescence is excited most efficiently in the range 670 - 715 nm.