Description
Optical Properties (in PBS, pH 7.4)
λ abs = 450nm
ε max = 4.5×104M-1 cm-1
λ fl = 495nm
η fl = 85%
CF260 = ε260/ε max = 0.31
CF280 = ε280/ε max = 0.30
Cyan 500 NHS-Ester is an amine-reactive fluorescent label. It is designed and recommended by Roche for application in LightCycler® instruments. The dye is used to label amino-modified oligonucleotides which can be detected on excitation at 430 nm in Roche LightCycler® instruments.
Cyan 500 NHS-Ester can also be used as label for other biomolecules such as proteins etc. The dye has a molecular weight of 580 g/mol and is moderately hydrophilic.