Description
Optical properties
λ abs = 625 nm
ε max = 1.4×10 5 M -1 cm -1
λ fl = 646 nm
n fl = 80 %
τ fl = 3.7 ns
CF 260 = ε 260 /ε max = 0.29
CF 280 = ε 280 / ε max = 0.46
ATTO Rho14 is a new rhodamine dye featuring a functionality for coupling to biomolecules such as DNA, RNA or proteins. The label shows strong absorption and extraordinarily high fluorescence quantum yield. In fact ATTO Rho14 is the brightest label available in this wavelength range. In addition the dye exhibits an exceptionally high photo-stability. In common with most ATTO-labels, absorption and fluorescence are pH-independent in the range of pH 2 to 11, used in typical applications.
The dye is moderately hydrophilic. After coupling to a substrate ATTO Rho14 carries a net electrical charge of +1. The fluorescence is excited efficiently in the range 605 - 640 nm . A suitable excitation source for ATTO Rho14 is the 633 nm line of the He:Ne laser or a diode-laser emitting at 635 nm.
