Leica, AD-RHO3B-35, ATTO Rho3B NHS-ester Fluorescent Label 5 mg

Optical properties
λ abs = 566 nm
ε max = 1.2×10 5 M -1 cm -1
λ fl = 589 nm
n fl = 50 %
τ fl = 1.5 ns
CF 260 = ε 260 /ε max = 0.27
CF 280 = ε 280 / ε max = 0.13
ATTO Rho3B is a new fluorescent label related to the well-known dye Rhodamine B. The dye shows strong absorption and high thermal and photo-stability. The characteristic feature of the dye is its temperature depending fluorescence yield. The dye is moderately hydrophilic. ATTO Rho3B is a cationic dye.
After coupling to a substrate the dye carries a net electrical charge of +1. In solution its fluorescence can be excited efficiently in the range 545 - 575 nm.