Leica, AD343H35, ATTO 343H NHS-Ester Hydrophilic Fluorescent Label 5 mg

Optical properties
λ abs = 343 nm
ε max = 1.9×10 4 M -1 cm -1
λ fl = 441 nm
n fl = 90 %
τ fl = 4.8 ns
CF 260 = ε 260 /ε max = 0.19
CF 280 = ε 280 / ε max = 0.15
ATTO 343H is a hydrophilic short wavelength fluorescent marker with a coumarin structure. The characteristic properties of this dye include high fluorescence quantum yield, large Stokes-shift, good photostability and low molecular weight. ATTO 343H s very water soluble and the fluorescence efficiency after coupling to biomolecules, such as a protein, is only slightly reduced even at higher degrees of labeling (DOL). ATTO 343H is an anionic dye. After conjugation to a biomolecule, the dye carries a single negative charge. Efficient excitation of fluorescence occurs in the 330 - 365 nm range.