Leica, AD553H35, ATTO 553H NHS-Ester Hydrophilic Fluorescent Label 5 mg

Optical properties
λ abs = 553 nm
ε max = 1.2×10 4 M -1 cm -1
λ fl = 573 nm
n fl = 85 %
τ fl = 3.6 ns
CF 260 = ε 260 /ε max = 0.27
CF 280 = ε 280 / ε max = 0.12
ATTO 553H is a fluorescent marker from the class of rhodamines. As a hydrophilic alternative to ATTO 550, the dye shows very good water solubility and a significantly reduced tendency to non-specific binding and dye aggregation. Like ATTO 550, ATTO 553H also has strong absorption, very high fluorescence quantum yield and high thermal and photochemical stability. The dye is therefore perfectly suited for use in single molecule detection and high-resolution microscopy (SIM, STED, etc.). ATTO 553H is an anionic dye. After coupling to a substrate, it carries a single negative charge. In contrast to ATTO 550, ATTO 553H is supplied in isomerically pure form. Efficient excitation of fluorescence occurs in the 540 - 565 nm range.