Description
Optical properties
λ abs = 576 nm
ε max = 1.2×10 5 M -1 cm -1
λ fl = 598 nm
n fl = 90 %
τ fl = 4.0 ns
CF 260 = ε 260 /ε max = 0.26
CF 280 = ε 280 / ε max = 0.10
ATTO 576H is a fluorescent marker from the class of rhodamines. As a hydrophilic alternative to ATTO Rho12, the dye shows very good water solubility and a significantly reduced tendency to non-specific binding. ATTO 576H possesses strong absorption, a very high fluorescence quantum yield, and high thermal and photochemical stability. The dye is therefore ideal for use in single-molecule detection and high-resolution microscopy (SIM, STED, etc.). ATTO 576H is an anionic dye. After coupling to a substrate, it is singly negatively charged. ATTO 576H is a mixture of three isomers with virtually identical absorption and fluorescence properties. Efficient fluorescence excitation occurs in the 550–585 nm range.
