Merck, 03-0196-00, SMC® Human Total α-synuclein High Sensitivity Immunoassay Kit

General description
α-Synuclein is a small, soluble protein predominantly found in the presynaptic terminals of neurons. It actively participates in synaptic function by regulating neurotransmitter release and maintaining synaptic integrity. Additionally, α-synuclein influences the dynamics of synaptic vesicles and interacts with various proteins involved in synaptic transmission. In neurological disorders, particularly neurodegenerative diseases like Parkinson′s disease, α-synuclein misfolds and aggregates into insoluble fibrils, forming Lewy bodies. These aggregates disrupt cellular function, leading to neuronal toxicity and death. The accumulation of α-synuclein also triggers neuroinflammation by activating microglial cells, which can further contribute to neurodegeneration.
Application
NeuroscienceNeurological DisordersNeurodegenerative DiseasesSynucleinopathies
Features and Benefits
Ultra-Sensitive Femtogram Analysis: Achieve exceptionally low detection limits (fg/mL) in serum, plasma and CSF.
High-Performance Signal Optimization: Reduced background and expanded dynamic range deliveres highly reproducible results.
Intuitive Assay Workflow: Utilize a familiar sandwich immunoassay protocol with next-generation sensitivity.
Scalable Automation: Integrate with robotic liquid handling systems to reduce hands on time and improve lab efficiency.
Expert Technical Support: Get end-to-end support from field scientists and technical support specialists.

Packaging
96 well assay
Analysis Note
Sensitivity: LLOQ 1,950 fg/mL* (SMCxPRO® Instrument)
Standard Curve Range: 490-500,000 fg/mL
Intra-assay CV: Inter-assay CV: Linearity: Serum: 113% Plasma: 118% CSF: 102%
Recovery: Serum: 96% Plasma: 96% CSF: 96%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Other Notes
Although Total α-Synuclein was detectable in non-human primate (rhesus macaque and cynomolgus monkey), feline, rat, mouse, and canine samples, the assay has not been verified for these species.
Legal Information
FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.