Description
General description
Synaptosomal-associated protein 25 (SNAP-25) plays a crucial role in synaptic transmission in the nervous system. As a component of the SNARE complex, it facilitates the fusion of synaptic vesicles with the presynaptic membrane, enabling the release of neurotransmitters into the synaptic cleft. By promoting efficient neurotransmitter release, SNAP-25 is essential for proper neuronal communication and signaling.
SNAP-25 has been implicated in various conditions, including Alzheimer′s disease, schizophrenia, and multiple sclerosis. Abnormalities in SNAP-25 expression or function can disrupt synaptic transmission, leading to impaired neuronal communication and contributing to cognitive decline and other symptoms associated with these disorders. Alterations in SNAP-25 levels often correlate with disease severity, highlighting its importance in neuroinflammation and synaptic dysfunction. Understanding the role of SNAP-25 in these processes can help identify potential therapeutic targets and inform strategies aimed at restoring synaptic function and improving outcomes for neurological disorders.
Application
Alzheimer′s Disease / Aging / Nervous System / Neurodegenerative Diseases / Neurofilament & Neuron Metabolism / Neuronal & Glial Markers / Neurodegenerative Medicine / Neuroscience
Features and Benefits
Ultra-Sensitive Measurement: Accurately detects SNAP-25 at femtogram per milliliter levels in serum, plasma and CSF.
Reliable Data: An improved signal-to-noise ratio extends the dynamic range to guarantee precise and trustworthy assay results.
Easy to Adopt: Follow a well-known sandwich immunoassay protocol to seamlessly incorporate SMC®technology into research processes.
Automated Workflow: Designed for compatibility with automated liquid handling systems, this assay minimizes manual tasks and enhances laboratory efficiency.
Expert Support: Get technical assistance from specialized field scientists and technical teams.
Packaging
96 well assay
Analysis Note
Sensitivity: LLOQ 260 fg/mL* (SMCxPRO® Instrument)
Standard Curve Range: 130-300,000 fg/mL
Intra-assay CV: Inter-assay CV: Linearity: Serum: 112% Plasma: 110% CSF: 97%
Recovery: Serm: 82% Plasma: 91% CSF: 89%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Other Notes
Although SNAP-25 was detectable in non-human primate (rhesus macaque and cynomolgus monkey), feline, rat, mouse, canine samples, the assay has not been verified for these species.
Legal Information
FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
