Merck, 1.09203, Giemsa′s azur-eosin-methylene blue

General description
Giemsa′s azur-eosin-methylene blue - for microscopy dye, is a dry dye that is used for the preparation of a staining solution. Giemsa′s staining is one of the standard procedures in histology, hematology, cytology and bacteriology. When used in hematological applications, Giemsa′s stain is frequently used in combination with other dye solutions, e. g. with May-Grünwald′s solution for Pappenheim (MGG) overview staining. The stain demonstrates various cells with their morphological features better than e.g. with H&E stain in histology. This staining solution generally stains the nuclei red, based on the molecular interaction between the Eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Application
For microscopy. For the staining of blood and bone-marrow smears, paraffin sections and clinical-cytological specimens
Analysis Note
Identity (UV/VIS-Spectrum)passes testLoss on drying (105 °C)≤ 10 %Suitability for microscopy (Blood smear)passes testNucleivioletLymphocytes (Plasma)blueMonocytes (Plasma)grey bluebasophilic granulocytes (Granules)dark violeteosinophilic granulocytes (Granules)reddish to red-brownNeutrophilic granulocytes (Granules)red-violetThrombocytesvioletErythrocytesreddish-brownishBlood parasites (Nuclei)lightred