Merck, 1.15938, Hematoxylin monohydrate (C.I.75290)

General description
Hämatoxylin-Monohydrat (C.I. 75290) for microscopy Certistain®, is a dye for the specific staining of DNA in the nuclei. It is used in cytology and histology. Nuclei are stained dark blue, dark violet to black. Hematoxylins are mixed with trivalent positively charged metal salts and build up the so-called hematoxylin lakes, used for the selective staining of nuclei (DNA). Certistain® is the quality group for seleceted high quality dyes that fulfill all quality requirements for IVD and CE registration. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Analysis Note
Dye content (spectrophotometrically): ≥ 95 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (acetonitrile): 290 - 292 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.05 g/l; acetonitrile): 155 - 165
Haematein (spectrophotometrically): ≤ 0.1 %
Water (according to Karl Fischer): 5 - 6 %
Suitability for microscopy: passes test
Legal Information
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany