Merck, 1083150003, TRIS hydrochloride

Name: TRIS hydrochloride
Brand: Merck
SKU: 1083150003

Size:300 g, Trizma® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride, EMPROVE® EVOLVE

Catalog Number: 1083150003

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Category

Biological Buffers

Pack Size300 g

TagsBiological Buffers Chemistry & Biochemicals Biochemicals

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

As part of our EMPROVE® Program, our raw materials are offered with EMPROVE® Dossiers which provide comprehensive, up-to-date documentation to help you navigate regulatory challenges, manage risks, and improve your manufacturing processes.

Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.

Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.
Application
Tris HCl is a commonly used biological buffer. Tris/Tris HCl as a buffering system has a very broad usage in biological systems for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.

Tris HCl is used as the conjugate buffer component with Tris/Tromethamine/Trizma in biopharmaceutical buffer formulations (both upstream and downstream applications), electrophoresis separations and diagnostic reagent formulations.
Other Notes
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Specifications

General

UNSPSC Code	12161700
Beilstein/REAXYS Number	3675235
MDL number	MFCD00012590
InChI key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
SMILES string	Cl.NC(CO)(CO)CO
InChI	1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
biological source	synthetic
description	Manufactured under appropriate controls for use as a raw material in BioProcessing
product line	EMPROVE® EVOLVE
form	powder
technique(s)	cell culture \| mammalian: suitable
impurities	Endotoxin, tested
useful pH range	7.0-9.0
pKa (25 °C)	8.1
suitability	suitable for manufacturing use
foreign activity	Cytotoxicity, contains (tested)
Quality Level	400

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