Merck, 11060102-DNA-5UG, B12.F8

Biochem/physiol Actions
Human B-lymphocyte cell line, immortalized with Epstein-Barr Virus (EBV), derived from a patient with chronic Hepatitis C (HCV type 1). Cell line produces human monoclonal antibody specific to the native HCV nucleoprotein N3. This antibody binds to native HCV nucleoprotein with no observable reactivity for the NS3, NS4 and NS5 regions. This antibody identifies a major B cell epitope within the immunodominant nucleoprotein amino terminal subregion (aa. 34-45). B12.F8 can be used in immunochemistry, HCV protein purification and HCV typing.CM3.B6 (Sigma product number 11060101) and B12.F8 were derived from the same patient as confirmed by STR profiling.
Human anti-hepatitis C virus IgG1 secreting B cell
STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6
TPOX: 9,11
vWA: 14,16

Preparation Note
RPMI 1640 (R0883) + 2 mM L-L-Glutamine (G7513) + 1% NEAA (M7145) + 1% NaP (S8636) + 10% FBS / FCS (F2442) + 20U/ml Interleukin-6 (IL-6).
Maintain cultures between 3-9 x 10⁵/ml. 5% CO₂; 37 °C. Media change every four days. Saturation density 1.1 x 10⁶/ml.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by any organization may require a license agreement. Customers will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].
Disclaimer
This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.