Merck, 158550-10G, Acridine Orange hemi(zinc chloride) salt

Name: Acridine Orange hemi(zinc chloride) salt
Brand: Merck
SKU: 158550-10G

Size:10 g, 3, 6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt, 3, 6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt), Basic Orange 14, Dye content 85 %

Catalog Number: 158550-10G

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Category

Special Stains

Pack Size10 g

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.
Acridine Orange hemi (zinc chloride) salt has been used to stain primary human umbilical vein endothelial cells (HUVEC) and is used in micronucleus tests (MNTs).
Biochem/physiol Actions
Acridine orange (AO) is a fluorescent dye with two different staining characteristics. In fixed tissues, it acts as a metachromatic dye and differentially stains DNA (green) and RNA (orange). But, in living cells it acts as a pH indicator and produce brilliant orange-red in acidic condition. AO has been used to identify apoptotic cells in drosophila embryos. Acridine Orange staining method has been used to deliver more consistent data in the micronucleus test.

Specifications

General

EC Number	233-353-6
UNSPSC Code	12171500
NACRES	NA.47
PubChem Substance ID	24849765
MDL number	MFCD00081043
Colour Index Number	46005
Beilstein/REAXYS Number	3734978
InChI key	RAHGLSRJKRXOSY-UHFFFAOYSA-L
InChI	1S/2C17H19N3.4ClH.Zn/c21-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h25-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
SMILES string	Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C
form	powder
composition	Dye content, 85%
solubility	H2O: 1 mg/mL, clear, orange to very deep orange
λ<sub>max</sub>	489 nm
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	room temp
Quality Level	200

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