Merck, 199664-25G, Sudan Black B

Name: Sudan Black B
Brand: Merck
SKU: 199664-25G

Size:25 g, Ceres black BN, Fat Black HB, Solvent Black 3, certified by the BSC

Catalog Number: 199664-25G

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Category

Microbiological Stains

Pack Size25 g

TagsClinical & Diagnostics Tissue Diagnostics Dry Dyes & Certified Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.
Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.
Biochem/physiol Actions
Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

Specifications

General

NACRES	NA.47
PubChem Substance ID	24851932
UNSPSC Code	12171500
EC Number	224-087-1
MDL number	MFCD00006919
Beilstein/REAXYS Number	723248
Colour Index Number	26150
InChI key	YCUVUDODLRLVIC-VPHDGDOJSA-N
InChI	1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+
SMILES string	CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46
agency	certified by the BSC
form	powder
technique(s)	microbe id \| staining: suitable
color	dark brown to very dark brown
mp	120-124 °C (lit.)
λ<sub>max</sub>	415 nm (2nd)
ε (extinction coefficient)	≥20000 at 596-603 nm in ethanol
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	room temp
Quality Level	200

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