Merck, 200220-100G, Azure II eosinate

Name: Azure II eosinate
Brand: Merck
SKU: 200220-100G

Size:100 g, Azure II-eosin; Methylene blue, eosinate of Azure II, powder

Catalog Number: 200220-100G

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Category

Special Stains

Pack Size100 g

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Azure II Eosinate is the eosinate of the dye Azure II which is a mixture of the dyes methylene blue and azure B in equal proportions. This compound is a key ingredient in Giemsa stain, which is widely utilized for staining blood elements and facilitating the visualization of cellular components under a microscope.
Application
Azure II Eosinate is used for human-medical cell staining and serves the purpose of the investigation of sample material of human origin (by fixing, embedding where necessary, staining, counterstaining, mounting) in hematological and clinico-cytological specimen materials, for example smears of whole blood and bone marrow.
Biochem/physiol Actions
The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azure B with DNA. Both dyes assemble to an Eosin Y- Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances.
Features and Benefits
Chief ingredient of Giemsa Stain.

Specifications

General

NACRES	NA.47
PubChem Substance ID	24851351
UNSPSC Code	12171500
MDL number	MFCD00084753
InChI	1S/C20H8Br4O5.C16H18N3S.C15H15N3S.2ClH.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;;;;/h1-6,25H,(H,27,28);5-10H,1-4H3;4-9H,1-3H3;21H;;/q;+1;;;;2+1/p-3
SMILES string	[Na+].[Na+].[Cl-].[Cl-].CNc1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C.CN(C)c4ccc5N=C6C=C\C(C=C6Sc5c4)=[N+](/C)C.[O-]C(=O)c7ccccc7C8=C9C=C(Br)C(=O)C(Br)=C9Oc%10c(Br)c([O-])c(Br)cc8%10
InChI key	CSDDIABEPMTRIP-UHFFFAOYSA-K
form	powder
technique(s)	microbe id \| staining: suitable
color	dark green
solubility	soluble, hazy to turbid
Quality Level	100
A<sub>max</sub>	≥0.50 at 644-655 nm in methanol at 0.005 g/L
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	room temp

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