Merck, 45346-250ML-F, Epoxy embedding medium, hardener DDSA
Size:250 mL, Dodecenylsuccinic anhydride, DDSA, suitable for microscopy
Catalog Number: 45346-250ML-F
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Category
Pack Size250 mL
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| MDL number | MFCD00005528 |
|---|---|
| UNSPSC Code | 41115710 |
| PubChem Substance ID | 24868811 |
| EC Number | 247-781-6 |
| NACRES | MA.02 |
| InChI | 1S/C16H26O3/c1-10(2)6-11(3)7-12(4)8-13(5)14-9-15(17)19-16(14)18/h8,10-12,14H,6-7,9H2,1-5H3/b13-8+ |
| InChI key | WBRBLKRDTHYRRV-MDWZMJQESA-N |
| SMILES string | CCCCCCCCC\C=C\CC1CC(=O)OC1=O |
| density | 1.00 g/mL at 20 °C |
| suitability | suitable for microscopy |
| application(s) | agricultureenvironmental |
| Quality Level | 100 |
Answer:Dodecenylsuccinic anhydride acts as an anhydride hardener in suitable epoxy resin formulations.
Answer:One bottle contains 250 mL of the DDSA hardener component.
Answer:No. DDSA is a curing component and must be formulated with epoxy resin and other required ingredients.
Sigma-Aldrich DDSA Epoxy Embedding Medium Hardener is dodecenylsuccinic anhydride, an anhydride curing component for preparing epoxy resin blocks used in microscopy. In an embedding formulation, DDSA reacts with the base epoxy medium in the presence of the specified catalyst or accelerator to create a crosslinked matrix. Its contribution to block hardness and sectioning behavior depends on the ratio of resin, DDSA and any second anhydride hardener such as MNA. The 250 mL bottle contains the DDSA component alone, not a complete embedding kit or a ready-cured mounting medium. Prepare the tissue by fixation and complete dehydration before resin infiltration. Measure each component accurately and mix thoroughly, because inconsistent proportions can produce soft, brittle or unevenly cured blocks. Perform the selected curing schedule under appropriate laboratory safety conditions and allow the block to stabilize before ultrathin sectioning. DDSA is chemically distinct from MNA; the two hardeners should not be treated as identical ingredients without adjusting the validated formulation.
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