Merck, 49641, Alcohol Dehydrogenase, recombinant from E. coli

Name: Alcohol Dehydrogenase, recombinant from E. coli
Brand: Merck
SKU: 49641

Size:, Alcohol:NADP⁺ oxidoreductase, ≥500 U/mL

Catalog Number: 49641

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Category

Recombinant Enzymes

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Research area: Neuroscience

Alcohol dehydrogenase has a homodimeric structure with a co-enzyme binding domain at the C-terminal and an N-terminal catalytic domain. The active site is located at the interdomain cleft. Binding of NAD+ in the active site causes conformational changes that create the binding site for the alcohol substrate.
Application
Alcohol dehydrogenase (ADH) has been used for the reversal of deficient 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay reduction in the disrupted schizophrenia 1 (DISC1-FL) and DB7 cell lysate.
Biochem/physiol Actions
Alcohol dehydrogenase catalyzes the oxidative conversion of alcohol into aldehyde. The metabolism of ethanol catalyzed by alcohol dehydrogenase (ADH) results in the generation of reactive oxygen species (ROS) and nitric oxide (NO) leading to oxidative damage to mitochondria and cellular proteins and is further associated with the onset of neuroinflammation and neurological disorders.
Other Notes
1 U corresponds to the amount of enzyme which reduces 1 μmol acetone per minute at pH 7.0 and 30°C (NADPH as cofactor)

Specifications

General

UNSPSC Code	12352204
NACRES	NA.54
Enzyme Commission (EC) Number	1.1.1.2 ( BRENDA \| IUBMB )
MDL number	MFCD00130451
recombinant	expressed in E. coli
form	liquid
specific activity	≥500 U/mL
technique(s)	cell based assay: suitable
color	light brownish-yellow to brown-green
suitability	suitable for molecular biology
application(s)	life science and biopharma
storage temp.	−20°C

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