Merck, 54457-250G-F, HEPES

Name: HEPES
Brand: Merck
SKU: 54457-250G-F

Size:250 g, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), BioUltra, Molecular Biology, ≥99.5% (T)

Catalog Number: 54457-250G-F

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Category

Biological Buffers

Pack Size250 g

TagsBiological Buffers Chemistry & Biochemicals Biochemicals

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Application
HEPES has been used as a component of:
DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells
RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)
Recapture solution to re-capture RNA exosomes from the glycerol gradient
ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation
2X HBS buffer that is used in transfection process for the production of recombinant lentivectors

Other Notes
3′-Terminal labeling of RNA with T4 RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer.
Easily compare specifications for HEPES products with the HEPES specification table.

Specifications

General

Web Name Suffix Override	≥99.5% (titration), powder, for molecular biology, BioUltra
NACRES	NA.25
PubChem Substance ID	57651473
UNSPSC Code	12161700
EC Number	230-907-9
MDL number	MFCD00006158
Beilstein/REAXYS Number	883043
eCl@ss	32129211
InChI key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
InChI	1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
SMILES string	OCCN1CCN(CC1)CCS(O)(=O)=O
grade	Molecular Biology
product line	BioUltra
assay	≥99.5% (T)
form	powder
impurities	DNases, none detected
ign. residue	≤0.1% (as SO4)
loss	≤0.5% loss on drying, 110 °C
pH	5.0-6.5 (25 °C, 1 M in H2O)
Quality Level	200
useful pH range	6.8-8.2
pKa (25 °C)	7.5
solubility	H2O: 1 M at 20 °C, clear, colorless
anion traces	chloride (Cl-): ≤50 mg/kg
cation traces	Al: ≤5 mg/kg

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