Description
General description
2-propanol is a clear, colorless, polar organic solvent commonly used in chemical and biochemical laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. Isopropanol is commonly used in molecular biology for DNA and RNA extraction through precipitation. Unlike ethanol, which requires 2.5 times the volume of the nucleic acid-containing sample, isopropanol can be added in an equal volume. This causes precipitation to occur even without cooling, making it advantageous in reducing the overall volume of the sample. Moreover, it has applications in quantifying hydroxyproline levels, assessing cell viability through MTT assays, and contributing to microarray hybridization processes.
Application
2-Propanol has been used:
to precipitate genomic DNA extracted from flies
to precipitate genomic DNA extracted from xenograft primary tumors and organs
to immunoprecipitate 5-bromo-2-deoxyuridine (BrdU)
Biochem/physiol Actions
Isopropanol (2-Propanol) is widely used in molecular biology for the precipitation of DNA during extraction from cells and tissues. BioUltra IPA is suitable for DNA precipitation.
Features and Benefits
Suitable as a reagent for Biochemical and Molecular Biology Research
BioUltra grade, meets strict purity standards through rigorous testing
Free from DNase, NICKase, RNase, and Protease
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This solvent is safe to use and thus aligns with "Safer Solvents and Auxiliaries". Click here for more information.
Other Notes
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