Merck, 68724-25MG, Fast Green FCF

Name: Fast Green FCF
Brand: Merck
SKU: 68724-25MG

Size:25 mg, Food green 3, analytical standard

Catalog Number: 68724-25MG

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Category

Polymer Standards

Pack Size25 mg

TagsChromatography Standards Analytical Chemistry Analytical Chromatography

CollectionsMerck Analytical Chromatography Products & Lab Supplies Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Fast Green FCF is a synthetic organic dye, which belongs to the triphenylmethane group. It can be found in a wide range of applications in food and beverage industry. It can also be used in scientific research for the staining of proteins and identification of extracellular recording or injection sites in electrophysiological experiments.
Application
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.

Specifications

General

UNSPSC Code	85151701
NACRES	NA.24
E Number	E143
PubChem Substance ID	329761116
EC Number	219-091-5
MDL number	MFCD00013053
Beilstein/REAXYS Number	5718212
Colour Index Number	42053
InChI	1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
SMILES string	[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
InChI key	RZSYLLSAWYUBPE-UHFFFAOYSA-L
grade	analytical standard
assay	≥98.0% (HPLC)
technique(s)	HPLC: suitable
mp	290 °C (dec.) (lit.)
application(s)	cleaning productscosmeticsfood and beveragespersonal care
format	neat
Quality Level	100

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