Merck, 85102263-1VL, AFRC MAC 1

Immunogen
2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii
Biochem/physiol Actions
Rat Y3Ag1.2.3 x Rat AO spleen
The antibody is directed against the glycoprotein 2BII. Binding is decreased when 2BII is boiled, and lost altogether after acid hydrolysis or de-glycosylation of 2BII.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].