Merck, 85102272-1VL, AFRC MAC 78

Immunogen
Nitrate reductase purified from the leaves of 6 to 8 week old spinach plants - Spinacea oleracea l
Biochem/physiol Actions
Rat YB2/3.0 Ag20 x Rat F344 spleen
The antibody is directed against NADH nitrate reductase of spinach and marrow. It inhibits the reduction of nitrate to nitrite using NADH as electron donor. It also inhibits the dehydrogenase activity of NADH-cytochrome C reductase.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].