Merck, 86052303-1VL, AFRC MAC 60

Immunogen
S-cis, trans-abscisic acid-4′-p-amino-benzoyl hydrazone-KLH conjugate
Biochem/physiol Actions
Rat Y3Ag1.2.3 x Rat LOU spleen
The antibody is directed against the plant growth substance cis, trans-abscisic acid. This hybridoma has been subcloned to produce AFRC MAC 252 (01071008). The antibody production rate of this hybridoma has been tested at ECACC. It must be noted that this hybridoma produces less than 2μg/ml of unpurified antibody.
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by [email protected] to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to [email protected].