Merck, 86120801-1VL, 3T6 Swiss Albino

General description
Product has lost contact inhibition
Biochem/physiol Actions
Derived from Swiss mouse embryos this cell line secretes collagen and hyaluronic acid. Unlike 3T3, the 3T6 line is not contact inhibited.
Mouse Swiss Albino embryo fibroblast
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000 to 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].