Merck, 93021844-DNA-5UG, C0155

Application
As part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.
Biochem/physiol Actions
A B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes. This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.
UK Caucasian Male
Preparation Note
Maintain cultures between 3 x 10⁵- 2 x 10⁶ cells/ml; 5% CO₂, 37 °C.
RPMI 1640 + 2 mM Glutamine + 20% Fetal Bovine Serum (FBS). Once a growing culture is established the FBS concentration can be reduced to 10%.
Other Notes
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