Merck, A2055-10EA, ACCUSPIN™ Tubes Sterile, 50 mL Capacity

General description
ACCUSPIN™ Tubes are polypropylene radiation sterilized tube fitted with a high density polyethylene barrier. Each tube will accept 15 mL of the density gradient.
Application
ACCUSPIN™ Tubes Sterile, 50 mL Capacity is intended for use with the density gradient separation medium Histopaque™ -1077:

in the isolation of lymphocytes and other mononuclear cells.
isolation of cord blood–derived endothelial progenitor cells.
isolate mononuclear cell layer from bone marrow samples.
suitable for the purification of human bone marrow aspirates for the investigation of the therapeutic value of BET inhibitors for acute myeloid leukemia.

Biochem/physiol Actions
Following the addition of Histopaque-1077 to theACCUSPIN Tube, a brief centrifugation places theHistopaque-1077 below the frit. The blood sample canbe added to the top chamber of the tube without risk ofmixing with the Histopaque-1077 in the lower chamberunder the frit. On subsequent centrifugation the wholeblood descends through the frit to contact with theHistopaque-1077. The elements of greater densitydisplace a volume of Histopaque-1077 above the fritgiving a clear separation of the blood components. Theerythrocytes aggregate and the granulocytes becomeslightly hypertonic, increasing their sedimentation rate,resulting in pelleting at the bottom of the ACCUSPINTube. Lymphocytes and other mononuclear cells, e.g.,monocytes, remain at the plasma/Histopaque-1077interface. This dense band of mononuclear cells maybe collected by pouring off the contents of the upperchamber or by means of a pipette. Erythrocytecontamination is avoided due to the barrier between thechambers.
Preparation Note
Package contains 10 tubes
Legal Information
Accuspin is a trademark of Sigma-Aldrich Co. LLC