Merck, A7481-200UL, Anti-Atg1/ULK1 antibody produced in rabbit

General description
Atg1 is an autophagy protein that functions as a serine/threonine kinase in yeast to regulate autophagasome formation. Atg1 is ULK1 in mammals.
Unc-51 like autophagy activating kinase 1 (ULK1) gene is located on human chromosome 12q24.33.
Immunogen
Synthetic peptide corresponding to amino acids of mouse Atg1/ULK1 conjugated to KLH via a cysteine residue. The corresponding sequence is identical in rat and differs by 3 amino acids in human.
Application
Anti-Atg1/ULK1 antibody produced in rabbit has been used in western blotting and immunohistochemical staining.
Biochem/physiol Actions
Anti- Atg1/ULK1 recognizes rat and mouse Atg1/ULK1.
Unc-51 like autophagy activating kinase 1 (ULK1) regulates autophagy initiation and innate immunity of interferons (IFNs). ULK1 is critical for neuronal development. It is essential for neurite elongation and differentiation of cerebellar granule neurons. Mutations in this gene is linked to latent mycobacterium tuberculosis infection (LTBI).
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.