Merck, A8301-25MG, Albumin, glycated human

General description
Albumin gene is mapped to human chromosome 4q13.3. It is synthesized as a preproalbumin with 609 amino acids in the liver. The mature albumin comprising 585 residues, is generated by the cleavage of signal peptide. Albumin is the major circulating protein in serum. It comprises three domains (I, II and III), each of which is subdivided into two subdomains A and B. A total of six ligand binding site is associated with albumin. Domain IIA and IIIA harbor small heterocyclic or aromatic compounds binding site. Long-chain fatty acid binding sites is present in IB and IIIB domains.
Application
Naturally occurring glycated albumin is a sensitive indicator of glycemic control by diabetic patients.
Albumin, glycated human has been used:
as a component of serum-free medium for retinal microglia activation
to stimulate immortalised proximal tubule epithelial (HK-2) cells for fibronectin production
as medium supplement in human retinal microvascular endothelial cells (HRMECs) cultures to test its effect on family with sequence similarity 18, member B (FAM18B) expression

Biochem/physiol Actions
Albumin is crucial for physiological processes and is the major transporter for lipids, metal ions and amino acids. It has antioxidant functionality and binds to drugs as well. Mutation in the albumin gene is implicated in an autosomal recessive disorder called Analbuminemia, which is characterized by low levels of albumin. The major glycation site is lysine 525 in albumin. Glycated albumin is a marker of glycemia in diabetes mellitus patients and is useful in assessment of HbA1c based glycemic control.
Physical form
Lyophilized powder containing citrate buffer salts
Preparation Note
Glycated in vitro
Other Notes
View more information on human serum albumin.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.