Merck, B0126-25G, Bromophenol Blue

Name: Bromophenol Blue
Brand: Merck
SKU: B0126-25G

Size:25 g, 3′, 3′′, 5′, 5′′-Tetrabromophenolsulfonephthalein, Bromophenol Blue (BPB), Bromphenol Blue Sultone Form, titration: suitable

Catalog Number: B0126-25G

Pricing

Send an RFQ.

View RFQ List

Category

Special Stains

Pack Size25 g

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Application
Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.

Specifications

General

Web Name Suffix Override	Powder
NACRES	NA.47
PubChem Substance ID	24891504
UNSPSC Code	12171500
EC Number	204-086-2
MDL number	MFCD00005875
Beilstein/REAXYS Number	61698
SMILES string	Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4
InChI	1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
InChI key	UDSAIICHUKSCKT-UHFFFAOYSA-N
agency	suitable for SM 2310
form	powder
technique(s)	titration: suitable
color	faint beige to very dark beige
visual transition interval	3.0-4.6, yellow-green to blue
mp	273 °C
solubility	methanol: 10 mg/mL
web metadata keyword.default	SM2310-SM2320
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	room temp
Quality Level	200

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.