Merck, B1256-25G, Bromocresol Green sodium salt

Name: Bromocresol Green sodium salt
Brand: Merck
SKU: B1256-25G

Size:25 g, 3′, 3″, 5′, 5″-Tetrabromo-m-cresolsulfonphthalein sodium salt, crystalline

Catalog Number: B1256-25G

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Category

Special Stains

Pack Size25 g

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
Bromocresol Green sodium salt has been used as a pH indicator for glutamate decarboxylase activity assay in Listeria monocytogenes strains.
Biochem/physiol Actions
Bromocresol Green is mainly used for measuring serum albumin. In presence of acidic conditions, bromocresol green interacts with albumin, thereby resulting in an increase in absorbance at 630nm. The change in absorbance is linked with the concentration of albumin. Bromocresol Green is also a pH indicator, changing from yellow (pH 4.0) to blue color (5.4).

Specifications

General

NACRES	NA.47
PubChem Substance ID	24891573
UNSPSC Code	12171500
Web Name Suffix Override	≥98% purity, crystalline
EC Number	263-657-4
MDL number	MFCD00148898
InChI key	HEFSGAHJDGZCHA-UHFFFAOYSA-M
InChI	1S/C21H14Br4O5S.Na/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21;/h3-8,26-27H,1-2H3;/q;+1/p-1
SMILES string	[Na+].Cc1c(Br)c(O)c(Br)cc1\C(=C2\C=C(Br)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O
agency	suitable for SM 2310
form	crystalline
visual transition interval	3.8-5.4, yellow to blue
mp	230 °C (dec.) (lit.)
solubility	H2O: 1 mg/mL
λ<sub>max</sub>	616 nm in H2O
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	room temp
Quality Level	200

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