| NACRES | NA.75 |
|---|---|
| UNSPSC Code | 12352207 |
| sterility | sterile-filtered |
| form | solution |
| technique(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| web metadata keyword.default | ACF grade bioreactor supplement, Serum-free medium, SFM, Chinese hamster ovary cell culture, biotechnology grade bioreactor supplement. |
| components | glucose: nophenol red: noL-glutamine: no |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Quality Level | 500 |
Answer:No. Neither glucose nor L-glutamine is included; each nutrient can be supplemented separately for the selected culture conditions.
Answer:It is a nutrient concentrate for fed-batch CHO bioreactor cultures and recombinant protein production.
Answer:The supplement is a sterile-filtered liquid supplied in 1 L, with storage at 2–8°C.
Sigma-Aldrich CHO Feed Bioreactor Supplement is a sterile-filtered liquid concentrate designed to replenish nutrients during fed-batch cultivation of Chinese hamster ovary cells. The animal component-free formulation contains amino acids, vitamins, recombinant human insulin, trace elements, and plant-derived hydrolysates to support extended CHO culture and recombinant protein expression. It does not contain glucose or L-glutamine, allowing those components to be managed separately in the bioreactor feed strategy. The ready-to-use supplement is supplied in a 1 L format and stored at 2–8°C. Feed amount and addition frequency depend on the CHO clone, base medium, viable cell density, and process objectives. The formulation is a nutrient supplement rather than a complete stand-alone basal cell culture medium.
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