Merck, CB1036-100UG, Anti-S100A7 Mouse mAb (1A4)

General description
Purified mouse monoclonal antibody. Recognizes the ~10 kDa S100A7 protein.
Recognizes the ~10 kDa S100A7 protein in A431 cells.
This Anti-S100A7 Mouse mAb (1A4) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of S100A7.
Immunogen
Full-length, recombinant, human S100A7 (aa 1-102)
Application

ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
Positive Control
A-431 cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)