Merck, CC130, PluriSTEM-XF® Recombinant Vitronectin

General description
PluriSTEM Recombinant Vitronectin is an effective xeno-free alternative to matrigel and has been validated to successully culture human ES and iPS cells using PluriSTEM Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.

PluriSTEM Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.
Application
Research Category
Stem Cell Research

Cell Structure

ECM & Adhesion
Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.
Physical form
Product is supplied in a 20 mM pH 8.0 Tris-HCl buffer with a proprietary formulation of sucrose, EDTA, arginine, and DTT.
Preparation Note
Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles. Product has been 0.2 μm filtered.
Analysis Note
Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).
Legal Information
PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany