Merck, CS0760-1KT, Isolated Mitochondria Staining Kit

Name: Isolated Mitochondria Staining Kit
Brand: Merck
SKU: CS0760-1KT

Size:1 kit, 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

Catalog Number: CS0760-1KT

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Category

mRNA Isolation Kits

TagsCell Analysis Cell Culture & Analysis Cell Isolation Kits

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.
Biochem/physiol Actions
The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.
Features and Benefits

Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

Analysis Note
The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

Specifications

General

UNSPSC Code	12352207
EC Number	217-896-6
NACRES	NA.32
usage	kit sufficient for 1000 reactions (using 96 multiwell plates)
packaging	pkg of 1 kit
storage condition	dry at room temperature
technique(s)	flow cytometry: suitable
fluorescence	λex 490 nm; λem 590 nm (JC-1 dye)
application(s)	cell analysisdetection
detection method	fluorometric
shipped in	dry ice
storage temp.	−20°C
Quality Level	200

Ordering

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