| NACRES | NA.25 |
|---|---|
| UNSPSC Code | 41105335 |
| form | liquid |
| usage | 100 uses |
| solubility | water: soluble |
| suitability | suitable for electrophoresis (DNA) |
| web metadata keyword.default | NA electrophoresis |
| storage temp. | −20°C |
| Quality Level | 100 |
Answer:The ladder contains 16 fragments from 50 bp to 10,000 bp.
Answer:No. Ficoll, bromophenol blue, and xylene cyanol are already incorporated in the loading solution.
Answer:Store at −20°C; the recommended gel loading volume is 10 µL per lane.
Sigma-Aldrich DirectLoad Wide Range DNA Marker provides a ready-to-load molecular-size reference for DNA gel electrophoresis. The aqueous ladder contains 16 DNA fragments spanning 50 to 10,000 base pairs and is premixed with a Ficoll-based loading solution containing bromophenol blue and xylene cyanol dyes. It can be applied directly to agarose gel wells without preparing a separate loading-dye mixture. The spread of fragment sizes supports estimation of PCR product lengths and analysis of DNA bands across a broad molecular-weight range. Agarose concentration can be adjusted to favor resolution of smaller or larger fragments, with approximately 0.75–2% agarose recommended for common applications. The reference is stored frozen at −20°C and is supplied as one vial with material for approximately 100 recommended 10 µL loads.
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