Merck, D9564-10MG, DAPI, dilactate

Name: DAPI, dilactate
Brand: Merck
SKU: D9564-10MG

Size:10 mg, 4′, 6-Diamidino-2-phenyindole, dilactate, ≥98% (HPLC)

Catalog Number: D9564-10MG

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Category

Special Stains

Pack Size10 mg

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.
Application
DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.
Biochem/physiol Actions
A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

Specifications

General

MDL number	MFCD00467366
UNSPSC Code	12171500
PubChem Substance ID	329798905
Web Name Suffix Override	≥98% purity (HPLC), powder
NACRES	NA.47
InChI key	LCAUPANIXBMNIA-UHFFFAOYSA-N
InChI	InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)
SMILES string	CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
assay	≥98% (HPLC)
form	powder
technique(s)	titration: suitable
color	yellow
solubility	H2O: 20 mg/mL
ε (extinction coefficient)	28.00-32.50 at 345-351 nm in methanol
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	2-8°C
Quality Level	300

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