Merck, D9785-10L, DMEM/Nutrient Mixture F-12 Ham

Name: DMEM/Nutrient Mixture F-12 Ham
Brand: Merck
SKU: D9785-10L

Size:10 L, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, DME/F-12, 1:1 mixture, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl₂, MgCl₂, MgSO₄, sodium bicarbonate, and phenol red, powder, suitable for cell culture

Catalog Number: D9785-10L

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Category

Cell Lysis Buffers

Pack Size10 L

TagsCell Culture & Analysis Cell Culture Media & Buffers Classical Media & Buffers

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labeL-tagged amino acids.
Preparation Note
Formulated to contain 14.8 grams of powder per liter of medium.
Supplement with 0.1545 g/L calcium chloride, 0.365 g/L L-glutamine, 0.05905 g/L L-leucine, 0.09125 g/L L-lysine, 0.0612 g/L magnesium chloride, 0.04884 g/L magnesium sulfate, 0.0172 g/L L-methionine, 1.2 g/L sodium bicarbonate.

Specifications

General

UNSPSC Code	12352207
NACRES	NA.75
Web Name Override	DMEM/F-12
Web Name Suffix Override	With HEPES, without ʟ-glutamine, ʟ-leucine, ʟ-lysine, ʟ-methionine, CaCl2, MgCl₂, MgSO4, sodium bicarbonate, and phenol red, powde, suitable for cell cutlure
MDL number	MFCD00217417
form	powder
technique(s)	cell culture \| mammalian: suitable
web metadata keyword.default	DME/Ham′s F-12, FBS, low-serum
components	L-glutamine: noHEPES: 15 mMsodium pyruvate: 0.055 g/LNaHCO3: nophenol red: noglucose: 3.15 g/L
shipped in	ambient
storage temp.	2-8°C

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