Description
General description
The CHEMICON in vitro angiogenesis assay kit represents a simple model of angiogenesis in which the induction or inhibition of tube formation by exogenous signals can be easily monitored. Angiogenesis is the process of generating new capillary blood vessels. It is a fundamental component of several normal (reproduction and wound healing) and pathological processes (diabetic retinopathy, rheumatoid arthritis, tumor growth, and metastasis).
The CHEMICON in vitro angiogenesis assay kit provides a convenient system for evaluation of tube formation by endothelial cells in a convenient 96-well format. When cultured on ECMatrix, a solid gel of basement proteins prepared from the Engelbreth Holm-Swarm (EHS) mouse tumor, these endothelial cells rapidly align and form hollow tube-like structures. Tube formation is a multi-step process involving cell adhesion, migration, differentiation, and growth.
ECMatrix is optimized for maximal tube-formation. For assaying inhibitors or stimulators of tube formation, simply premix the endothelial cell suspension with different concentrations of the inhibitor or stimulator to be tested, before adding the cells to the top of the ECMatrix.
Application
In vitro angiogenesis assay kit has been used to monitor the extent of tube assembly/angiogenic potential in various endothelial cells such as:
human umbilical vein cells (HUVEC)
primary human retinal microvascular cells
platelet derived growth factor receptor-β (PDGFRβ)/PAE cells
bovine capillary endothelial (BCE) cells
Preparation Note
Store kit materials at -20 ºC for up to 6 months. Once ECMatrix has been thawed, store at 2-8 ºC for one week. Do not refreeze.
Other Notes
ECMatrixTM Gel Solution: (Part No. 90060) Five 1 mL bottles.
ECMatrixTM Diluent Buffer, 10X: (Part No. 90061) One 1 mL bottle.
The CHEMICON in vitro angiogenesis assay kit is intended for research use only; not for diagnostic or therapeutic applications.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
