Merck, F0162, Fibronectin Proteolytic Fragment from human plasma

Name: Fibronectin Proteolytic Fragment from human plasma
Brand: Merck
SKU: F0162

Size:, Fibronectin, lyophilized powder, 45 kDa

Catalog Number: F0162

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Category

General Purpose Incubators

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface.FN1 is mapped to human chromosome 2q35. Proteolytic fragments of fibronectin has a cell binding domain. Plasma fibronectin has two polypeptide chains connected by two disulphide bonds present near carboxy terminal. Each polypeptide chain is of 220-250 kDa.
Application
Fibronectin proteolytic fragment from human plasma has been used as fluorophore-conjugated proteins. It has also been used in solid phase binding assay.
Biochem/physiol Actions
Fibronectin participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Proteolytic fragments of fibronectin plays a vital role in mononuclear phagocyte function.
Fibronectins are high molecular weight glycoproteins with two subunits joined by a disulfide bond to form the dimer. The fragments are obtained using protelytic enzymes. This 45 kDa gelatin binding fragment is obtained through trypitc digestion of the N-terminal 70 kDa fragment, which is produced by Cathespin D digestion.

This fragment has an acidic pI (4.9-5.3) and does not bind to heparin. This domain is resistant to proteolysis due to intrachain disulfide bonding and the attached carbohydrate. The intrachain disulfide bonds are essential for binding to gelatin, while the complex, branched, asparagine-linked carbohydrate is not. This fragment binds to C1q, but not to fibrin.
Preparation Note
This product is lyophilized from phosphate buffered saline with sucrose as a cryoprotectant. The source material has tested negative for antibody to HIV, HCN, and HBsAg. It is soluble in water at 0.5 mg/mL and yields a clear to slightly hazy solution.
Disclaimer
The product should be stored at -20°C.

Specifications

General

UNSPSC Code	12352202
eCl@ss	42020141
NACRES	NA.75
MDL number	MFCD00131062
biological source	human plasma
assay	≥90% (SDS-PAGE)
form	lyophilized powder
mol wt	45 kDa
packaging	pkg of 0.5 mg
technique(s)	cell culture \| mammalian: suitable
impurities	HIV and HBsAg, source material tested negative
solubility	water: soluble ≥0.500 mg/mL, clear to slightly hazy, colorless
UniProt accession no.	P02751
shipped in	wet ice
storage temp.	−20°C
Quality Level	200

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