Merck, F0500-25G, Fast Blue RR Salt

Name: Fast Blue RR Salt
Brand: Merck
SKU: F0500-25G

Size:25 g, 4-Benzoylamino-2, 5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt, Azoic Diazo No. 24, crystalline

Catalog Number: F0500-25G

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Category

Special Stains

Pack Size25 g

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
Fast blue RR salt has been used in the immunohistochemistry to detect the activity of alkaline phosphatase. It has been used in the procedure of connective tissue staining using picrosirius red.
Biochem/physiol Actions
Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appears black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.

Specifications

General

Web Name Suffix Override	crystalline
NACRES	NA.47
PubChem Substance ID	24894722
UNSPSC Code	12171500
EC Number	238-777-5
MDL number	MFCD00074762
Colour Index Number	37155
InChI key	LLARVKAOQLUCQI-UHFFFAOYSA-L
InChI	1S/2C15H13N3O3.4ClH.Zn/c21-20-13-9-12(18-16)14(21-2)8-11(13)17-15(19)10-6-4-3-5-7-10;;;;;/h23-9H,1-2H3;4*1H;/q;;;;;;+2/p-2
SMILES string	[Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1NC(=O)c2ccccc2.COc3cc([N+]#N)c(OC)cc3NC(=O)c4ccccc4
form	crystalline
technique(s)	microbe id \| staining: suitable
solubility	water: 1 mg/mL, clear to hazy, yellow-green
ε (extinction coefficient)	≥15000 at 326-332 nm
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	−20°C
Quality Level	200

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