Merck, F2518-.5MG, Fibronectin human foreskin fibroblasts

Name: Fibronectin human foreskin fibroblasts
Brand: Merck
SKU: F2518-.5MG

Size:0.5 mg, Fibronectin, lyophilized powder, suitable for cell culture

Catalog Number: F2518-.5MG

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Category

Differentiation Factors

Pack Size0.5 mg

TagsCell Culture & Analysis Cell Culture Supplements & Reagents Attachment Factors

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Fibronectin is mostly synthesized by fibroblasts and endothelial cells.It is an adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms.
Application
Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay and probiglycan and cleavage assays. It has also been used in ectoderm (neuronal) differentiation and in the preparation of a semi-solid growth medium.
Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells
Biochem/physiol Actions
Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.
Features and Benefits

Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium.
It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts.
The starting material is tested and found negative for HIV and HBsAg.
The product is soluble in sterile water, yielding a clear, colorless solution.
It can be stored at -20°C for at least three years.

Physical form
Lyophilized in CAPS buffered saline
Preparation Note
Store reconstituted solution in working aliquots at -20°C or lower.
dialyzed against chloroform
Analysis Note
Protein homogeneity is evaluated by immunoelectrophoresis.
Disclaimer
Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

Specifications

General

UNSPSC Code	12352202
NACRES	NA.75
EC Number	289-149-2
Web Name Suffix Override	from human foreskin fibroblasts, powder, suitable for cell culture
Web Name Override	Human Fibronectin
MDL number	MFCD00131062
biological source	human foreskin fibroblasts
product line	BioReagent
form	lyophilized powder
mol wt	~550 kDa
packaging	pkg of 0.5 mg
technique(s)	cell culture \| mammalian: suitable
surface coverage	1‑5 μg/cm2
impurities	Human Source, none detected (HCV)
solubility	H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)
web metadata keyword.default	attachment factor, mesenchymal cell attachment, neuronal cell attachment, fibroblast attachment, neural crest cell attachment, endothelial cell attachment
NCBI accession no.	NM_212482.4">NM_212482.4
UniProt accession no.	P02751
binding specificity	Peptide Source: Collagen
shipped in	ambient

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