Merck, H2779-1L, HistoChoice® Clearing Agent

General description
HistoChoice® is a patented, complex mixture of agents that allow tissues to retain their structure, antigenic sites, and nucleic acid sites. It is a non-toxic, non-cross-linking fixative formulated to replace formaldehyde-based fixation. This formulation in non-citrus, contains no mercury, formaldehyde, or glutaraldehyde.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of formalin for tissue processing.
Application
HistoChoice® Clearing Agent has the following applications:It is suitable as an alternative to toluene and xylene for dewaxing paraffin tissue sections. Prepared slides are suitable for antibody probing applications and in situ hybridizations.
It has been employed in a study to explain the recalcitrance of lignocellulosic plant cell walls to enzymatic degradation.
It has been used to develop a novel method to improve sensitivity for the localization of miRNA and their target transcripts in plants.
It has been used to study the correlation of cytoplasmic ADP-ribosylation levels with markers of patient outcome in human cancers.

Biochem/physiol Actions
Fixation with HistoChoice® works well in immunohistology applications where its non-cross-linking traits are conducive to epitope retrieval and identification by primary antibodies to extracellular matrix components.
Features and Benefits
Non-toxic, non-carcinogenic, and odorless.
Non-flammable.
Preserves native antigenic and nucleic acid sites.
Compatible with many organic mounting media.
Dissolves wax faster and evaporates slower than xylene.
Can be used in automated processors.
Tissues retain their original structure.

Legal Information
HistoChoice is a registered trademark of Amresco, Inc.