Merck, H4290-1MG, Hydroxyphenyl fluorescein solution

Name: Hydroxyphenyl fluorescein solution
Brand: Merck
SKU: H4290-1MG

Size:1 mg, Hydroxyphenyl fluorescein, 2-[6-(4′-hydroxy)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid, HPF, 5 mM in DMF

Catalog Number: H4290-1MG

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Category

Microbiological Stains

Pack Size1 mg

TagsClinical & Diagnostics Tissue Diagnostics Dry Dyes & Certified Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Hydroxyphenyl fluorescein solution, also known as HPF is a fluorescent probe, which upon oxidation by the hydroxyl radical (HO) returns a brilliant green fluorescence (HPF-ox). Increased HPF signals in antibiotic-exposed bacterial cells are explained by fluorescence associated with increased cell size, not by ROS levels. In cells incubated with the HPF probe and exposed to ampicillin and norfloxacin with HPF fluorescence correlated linearly.
Application
Fluorescent reagent for the detection of highly reactive oxygen species (hROS). Fluorescence intensity increases after the immediate reaction with hROS such as hydroxyl radical and peroxynitrite. Since HPF does not react with nitric oxide, superoxide and hydrogen peroxide, it can differentially detect peroxynitrite. HPF is resistant to light-induced autoxidation.
Hydroxyphenyl fluorescein solution is used to detect highly reactive oxygen species (hROS). It is a suitable probe to detect cellular death caused by bacterial antibiotics.
Features and Benefits
The following are the benefits of Hydroxyphenyl fluorescein solution:

HPF is resistant to light-induced autooxidation.
Hypochlorite(-OCl) does not react with HPF.
HPF does not react with nitric oxide, superoxide, and hydrogen peroxide.
HPF has low reactivity with HOCl.

Specifications

General

UNSPSC Code	12352108
EC Number	200-679-5
NACRES	NA.32
concentration	5 mM in DMF
fluorescence	λex 490 nm; λem 515 nm
storage temp.	2-8°C
Quality Level	200

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