Merck, LGC602402-1KIT, Endura Chemically Competent Cells (DUOs)

Name: Endura Chemically Competent Cells (DUOs)
Brand: Merck
SKU: LGC602402-1KIT

Size:12 x 80 μL, Heat shock competent cells, DNA cloning, DNA transformation, competent E.Coli, pkg of 24 reactions (12 x 80 µL), Cfu/µg > 1 x 10⁷

Catalog Number: LGC602402-1KIT

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Category

Competent Cells

Pack Size1 Kit

TagsLabware Labware Partners LGC Biosearch™ Products

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.
Application
Cloning and maintaining unstable or repetitive DNA sequences.
Recommended for high efficiency propagation of GeCKO CRISPR libraries (1)
Ideal for single-guide RNA lentiviral library construction (2)

Features and Benefits
Clone repetitive sequences and lentiviral libraries
Stabilize direct repeats and create lentiviral constructs

Transformation efficiency: ≥ 1 x 107 (cfu/µg)

Genotype: Endura: recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

Components:

Endura Chemically Competent Cells

pUC 19 Trasformation Control

Recovery Media

Specifications

General

UNSPSC Code	41106202
NACRES	NB.43
biological source	Escherichia coli
manufacturer/tradename	Lucigen 60240-2
packaging	pkg of 24 reactions (12 x 80 µL)
growth mode	growth or suspension
technique(s)	microbiological culture: suitable
cell transformation	competent cell type: chemically competenttransformation efficiency: 1×107 cfu/μg
shipped in	dry ice
storage temp.	-65 to -96°C

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