Description
General description
112.7 kDa (alpha-V) and 86.34 kDa (beta-5) calculated. ~150 kDa (alpha-V) and 95 kDa (beta-5) reported (Weinacker, A., et al. (1994). J. Biol. Chem. 269(9):6940-6948) due to glycosylation.
Integrin alpha-V (UniProt P06756; CD51, Integrin alpha V, Vitronectin receptor subunit alpha) and Integrin beta-5 (UniProt P18084) are encoded by the ITGAV (MSK8, VNRA, VTNR; Gene ID 3685) and the ITGB5 (Gene ID 3693) gene, respectively, in human. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and a beta subunit associated in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence present in the extracellular matrix components, such as fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Integrin alphaVbeta5 is the primary mechanotransducer that mediates lung myofibroblast contraction-induced latent TGF-beta1 activation. Increased alphaVbeta5 expression is reported to be associated with myofibroblast differentiation. Integrin alphaVbeta5 is shown to interact with Thy-1 in an RLD-dependent manner. Thy-1 expression prevents fibroblast contraction-induced latent TGF-beta1 activation and lung myofibroblast differentiation.
Immunogen
Epitope: Extracellular region.
Human UCLA P3 lung carcinoma cells (Weinacker, A., et al. (1994). J. Biol. Chem. 269(9):6940-6948).
Application
Anti-Integrin αVβ5 Antibody, clone P1F6, Phycoerythrin conjugate is an antibody against Integrin αVβ5 for use in Flow Cytometry.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
The unconjugated antibody (Cat. No. MAB1961 & MAB1961Z) is shown to be suitable also for Flow cytometry, Functional blocking, Immunocytochemistry, Immunohistochemistry, and Immunoprecipitation applications.
Biochem/physiol Actions
Clone P1F6 recognizes αVβ5 without cross-reactivity toward αVβ3 (Weinacker, A., et al. (1994). J. Biol. Chem. 269(9):6940-6948).
Physical form
Protein A purified
Purified mouse monoclonal IgG1 antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Flow Cytometry in HT1080 cells.
Flow Cytometry Analysis: 1.0 µg of this antibody detected Integrin αVβ5 in HT1080 cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
