Merck, MAK117-1KT, Glycerol Assay Kit

The Sigma-Aldrich Glycerol Assay Kit measures free glycerol in biological samples, foods, beverages, and pharmaceutical formulations using a coupled enzymatic reaction. Glycerol kinase and glycerol phosphate oxidase generate a detectable reaction product with a signal proportional to glycerol concentration. The assay supports colorimetric measurements at 570 nm across 10–1,000 µM glycerol and fluorometric measurements at approximately 535 nm excitation and 587 nm emission across 2–50 µM. The kit provides enough reagents for approximately 200 tests in a 96-well format and includes an assay buffer, dye reagent, enzyme mix, glycerol standard, and ATP. A suitable plate reader and microplates are required separately. Biological culture supernatants, plasma, and formulated samples may require dilution or matrix-specific optimization before measurement. Store reagents at −20°C, protect from light, and minimize repeated freeze–thaw cycles. Colorimetric and fluorescence workflows share the enzymatic principle but have different working concentration ranges.