Merck, P2714-1BTL, Protease Inhibitor Cocktail powder

General description
The protease inhibitor cocktail has been optimized and tested for general use. It consists of water-soluble protease inhibitors that effectively inhibit serine, cysteine, and metalloproteases. This product is supplied as a lyophilized powder.
Application
Protease Inhibitor Cocktail powder has been used as a component:
in radioimmunoprecipitation assay buffer to lyse the avian crystalline lens samples for western blot analysis
in phosphate buffer solution (PBS) for grinding recombinant protein expressed leaves to purify virus-like particles (VLPs)
in PBS to dissolve lyophilized skin secretion sample of A. loloensis for peptide purification
in 0.1 M NaCl solution to wash and collect skin secretions of frog

Biochem/physiol Actions
Inhibits serine, cysteine, and metallo-proteases.
The components of this mixture, such as AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.
Preparation Note
One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.
Other Notes
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
This mixture contains individual components, including:

AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM

Each component has specific inhibitory properties.
Disclaimer
The lyophilized powder is stable for 4 years when stored at -20°C.