Description
General description
EMD Millipore′s Human ES/iPS Neural Induction Mediium is an serum free/cheimcally defined optimized media for the rapid production of expandable neural progenitor cells from pluripotent human ES and iPS cells. The media relies on both small molecules neural inducers and supplements to generate a highly enriched population of expandable neural progenitors based on establish protocols. Neural progenitors are generated 10 days from starting cultures of traditional feeder-based and/or feeder-free cultures of undifferentiated human ES/iPS cells and can be expanded for over 3-5 passages resulting in a minimum 20-fold expansion. Typically, from 1 well of a 6-well plate containing an estimated 500,000 pluripotent human ES/iPS, approximately 5-10 million neural progenitors can be obtained after 3 passages. The resulting neural progenitor cells are multipotent and can be further differentiated to specific neural cell subtypes of interest.
Preparation Note
Store at -20C. Good for 3 months from date of receipt or until expiration date on bottle when reagents are handled and stored appropriately. Please note: The supplemented ES/iPS Neural Induction Medium should be stored at 2 to 8C for up to 4 weeks.
Other Notes
1) Neural Induction Basal Medium (Part No. CS210991). One 125 mL bottle. 2) Neural Supplement 1 (50X) (Part No. CS210992). One 2.5 mL bottle. 3) Neuro2 Medium Supplement (Part No. SCM012-S). One 1.25 mL bottle. 4) GSK3 and TGFbR Inhibitor Cocktail (1000X) (Part No. CS210983-150UL). One 150 µL vial. 5) AMPK Inhibitor (1000X) (Part No. CS210995-60UL). One 60 µL vial.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
